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APP is endogenously synthesized in a cap-independent manner in rat C6 glioma cells. C6 cells in culture were treated for 16 h with either DMSO or rapamycin. Cell lysates were analyzed via western blotting for (A) APP, phosphorylated p70s6 kinase and total p70s6 kinase, (B) connexin37 and GAPDH as a loading control. Imagequant Software was used to quantify APP expression from six separate experiments.
