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The APP 5′-leader demonstrates IRES activity in a monocistronic context. Monocistronic Photinus reporter mRNA with the β-globin or APP 5′-leader downstream of either an m7G cap or ApppG cap were co-transfected with monocistronic m7G-capped humanized Renilla luciferase mRNA, to normalize for transfection efficiency, into C6 cells. Following a 7 h transfection into C6 cells, Photinus and Renilla luciferase activities were assayed.
