Figure 8.

ex vivo conditions which mimic the AD brain state lead to an increase in cap-independent translation of APP. Rat neural C6 cells were either treated with FAC for 24 h or acutely with chemical ischemia reagents (sodium azide and dideoxyglucose), for 10 min, 7 h prior to harvest both treatments carried out in the absence or presence of rapamycin for 16 h. Cell lysates were probed for (A & B) APP, phospho-p70s6 kinase and GAPDH levels using Western blotting. (C) APP levels were quantified using Molecular Dynamics Imagequant software and normalized for protein loading. APP protein levels were normalized to DMSO (untreated) controls. Data graphed represent five separate experiments.