Figure 9.

Alignment extension with PfFSmat60 for P. falciparum bi-functional enzyme of the shikimate pathway. The sequences compared here are the P. falciparum hypothetical protein, PFB0280w and yeast multifunctional protein, P08566. (a) The alignment yielded with BLOSUM100, showing no alignment overlap for the motif regions, EPSP synthase I and shikimate kinase. (b) The alignment with BLOSUM50 showing the aligned motif regions for only EPSP synthase I motif (gray shading). (c) The alignment extended by PfFSmat60 for both the EPSP synthase I and shikimate kinase motifs represented as (i) and (ii), respectively. The text shaded in gray corresponds to the EPSP synthase I motif. The text in white with dark gray shading represents the shikimate kinase motif. PFB0280w gave only hypothetical hits with BLASTp at the default parameters.