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Schematic representation of PCR amplicon and plasmid pHWSccdB. The two strands of the PCR amplicon serve as megaprimers. Each 3′-end of the megaprimer anneals to the complementary annealing site within the plasmid pHWSccdB and is elongated during target-primed plasmid amplification leading to replacement of the ccdB marker by the viral gene. The two newly synthesized strands hybridize to nicked circular molecules.
