Figure 3.

Purified PCR amplicons used as megaprimers for target-primed plasmid amplification. For each lane, three PCR assays (each 100 µl) were pooled and agarose-gel purified (total volume 50 µl), except for PB1 for which only one assay was used. Lanes: S DNA size marker (3 µl) and purified amplicons (each 5 µl).