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The dual control of TLS by REV1 and PCNA ubiquitination. (A and B) Replication efficiency of the pQTs and pQTo shuttle plasmids in xpa, xpa rev1, xpa rad18 and xpa pcnaK164R cells (A), and in wt and rev1 pcnaK164R cells (B), normalized against an internal pQ2 control as in Figure 2. The average and SEM of 3–5 experiments is shown. (C) The proportion of TLS versus error-free bypass in pQTs sequences recovered from the indicated cell lines, shown as a percentage of the total.
