Figure 5. Identification of σ1 residues required for infection of cultured cells and JAM-A binding.

(A) Infection of HeLa cells by parent or mutant virus. Cells were treated with either PBS alone (mock) or 40 mU/ml of neuraminidase diluted in PBS (NA). Infected cells were identified by indirect immunofluorescence and quantified in three fields of view. The results are expressed as the mean fluorescent focus units (FFU) per field for triplicate experiments. Error bars indicate standard deviations. The asterisk indicates P<0.05 in comparison to the control. (B) SPR analysis of reovirus binding to JAM-A. Traces show binding and dissociation from GST-JAM-A for purified reoviruses at a concentration of 6×10¹² (green), 8×10¹² (orange), and 10¹³ (red) particles/ml. Reovirus binding to the capturing molecule (grey) is set as the baseline. Binding is expressed in resonance units (RU).