Table 2. Primers for PCR (* for q-PCR).
| Transcript | Primer sequence (Forward/Reverse) | Tm | Product size |
| Oct3/4 | CAATGCCGTGAAGTTGGAGA | 60 | 81 |
| GCTTCAGCAGCTTGGCAAAC | |||
| Nanog | TTTCAGAAATCCCTTCCCTCG | 60 | 81 |
| CGTTCCCAGAATTCGATGCT | |||
| Sox2 | AAGGGTTCTTGCTGGGTTTT | 60 | 150 |
| AGACCACGAAAACGGTCTTG | |||
| Brachyury T | AGGCTCCCCTGCACATTACA | 60 | 71 |
| AGCAGCCCCTTCATACATCG | |||
| Cdx2 | GGGTGGGGGTAGCAATACTT | 60 | 82 |
| TGCCTCTGGCTCCTGTAGTT | |||
| NKX2.5 | CGACGGAAGCCACGCGTGCT | 60 | 181 |
| CCGCTGTCGCTTGCACTTG | |||
| GATA4 | TCTCACTATGGGCACAGCAG | 60 | 246 |
| CGAGCAGGAATTTGAAGAGG | |||
| α-MHC | CTGCTGGAGAGGTTATTCCTCG | 64 | 301 |
| GGAAGAGTGAGCGGCGCATCAAGG | |||
| Mlc2a | CAGACCTGAAGGAGACCT | 52 | 286 |
| GTCAGCGTAAAACAGTTGC | |||
| Mlc2v | TGTGGGTCACCTGAGGCTGTGGTTCAG | 60 | 189 |
| GAAGGCTGACTATGTCCGGGAGATGC | |||
| Sox1 | AGATGGCCCAGGAAAACCC | 60 | 178 |
| CCTCGGACATGACCTTCCAC | |||
| Sox17 | TCCCTACCAGGGACACGACT | 60 | 194 |
| GAGCTAGCGTCGGACACCAC | |||
| MyoD | ATGCTGGACAGGCAGTCGAGGC | 65 | 144 |
| GCTCTGATGGCATGATGGATTACAGCG | |||
| vWF | CCACTTGCCACAACAACATC | 60 | 150 |
| TGGACTCACAGGAGCAAGTG | |||
| β-Tubulin | GAAGAGGAGGCCTAACGGCAGAGAGCCCT | 60 | 182 |
| GAGTGCCTGCCATGTGCCAGGCACCATTT | |||
| ANF* | TGATAGATGAAGGCAGGAAGCCGC | 64 | 203 |
| AGGATTGGAGCCCAGAGTGGACTAGG | |||
| Calsequestrin* | TGAACTTCCCCACGTACGATG | 60 | 307 |
| AAACTCAATCGTGCGGTCACC | |||
| cTnI* | AGGGCCCACCTCAAGCA | 58 | 103 |
| GGCCTTCCATGCCACTC | |||
| ssTnI* | GCACTTTGAGCCCTCTTCAC | 60 | 169 |
| GTGTTCCTGCTCCCAACACT | |||
| Rb* | CCTTGAACCTGCTTGTCCTC | 60 | 191 |
| GGGCAAGGGAGGTAGATTTC | |||
| P107* | GATGCTCATCTGACCGGAGT | 60 | 83 |
| ATAAGTCACGTAGGCGCACA | |||
| P130* | GGAAATGCCCTTCAGTGTTC | 60 | 101 |
| GGACGCTTTAGAGTCCTTGG | |||
| E2F1* | ATGGAAGAGGACCAACTGTC | 60 | 230 |
| CCTGAATCCCTAGGCTTCTG | |||
| E2F2* | TAGGGAGATGTGGAGGATTCGG | 60 | 206 |
| AACTCAGGGTGGACAAACAAACAC | |||
| E2F3a* | GCCTCTACACCACGCCACAAG | 60 | 99 |
| TCGCCCAGTTCCAGCCTTC | |||
| E2F4* | CTTCTACCTCCTTTGAGCCCATC | 60 | 96 |
| TCACAGACACCTTCACTCTCGTCC | |||
| E2F5* | ACCTGATGACCTCACACAGCCTTC | 60 | 202 |
| GGGGTAGGAGAAAGCCGTAAAAG |