Figure 7.

A) Immobilization of 0.5 mg/mL HyNic-functionalized streptavidin from PBS buffer (blue) and from 10% (v/v) rat plasma diluted in PBS (green). B) Immobilization of 0.1 mg/ml biotinylated BSA from PBS buffer (blue) and from 10% (v/v) rat plasma diluted in PBS (green) onto a 40% saturated streptavidin surface. C) Immobilization of 0.1 mg/ml biotinylated BSA from PBS buffer (blue) and from DIMEM medium supplemented with 5% (v/v) fetal bovine calf serum (green) onto a 40% saturated streptavidin surface. 50 μL sample and reference volumes each were exposed to the sensor surface over 500 seconds.