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The sequence of the primers used to amplify 5-HT1 receptor mRNA from PAG tissue.
| Receptor | Product size (bp) | Primers |
| 5-HT1A | 113 | 5'-TCTGTTGCTGGGTACTCTCA-3' |
| 5'-AGGAGCCGATGAGATAGTTG-3' | ||
| 5-HT1B | 151 | 5'-ATGGAGGAGCAGGGTATTCA-3' |
| 5'-AAGCAACCAGCAGGACTTTC-3' | ||
| 5-HT1D | 171 | 5'-ATCTCAGATTTCTTACACCATCTACTC-3' |
| 5'-CGCAGAGCCCGTGATAAG-3' | ||
| 5-HT1F | 108 | 5'-ACCACCCAGCCAACTATTTA-3' |
| 5'-TAATCCAACTCTCGCTCACA-3' |
For each receptor the forward and reverse primers are shown, respectively. bp is base pairs.
