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Reverse-transcriptase PCR analysis of selected genes involved in multiple pleiotropic drug resistance. (A) Semi-quantitative RT-PCR conducted with RNA extracted after 24 hr of induction from EPY307 (artemisinic acid producer) and EPY309 (amorphadiene producer). Number of PCR Cycles used: ACT1: 23; PDR5, PDR15, SNQ2: 24; YOR1:27; PDR1:28; PDR3, PDR12:30. (B) Quantitative real-time RT-PCR conducted with samples harvested at 24 and 96 h post-induction.
