Table 2.

Brachypodium reference genes for normalization and their expression stability values calculated by the NormFinder software

Rank	Tissue		Growth hormone		Heat/Cold		High salt/Drought		Developmental stage		Total
	Gene	Stability	Gene	Stability	Gene	Stability	Gene	Stability	Gene	Stability	Gene	Stability
1	Ubi4	0.0846	Ubi10	0.1377	GAPDH	0.1723	SamDC	0.0372	Ubi4	0.0987	UBC18	0.2586
2	Ubi10	0.0846	Ubi4	0.1640	EF1α	0.1951	UBC18	0.0378	SamDC	0.0996	Ubi4	0.3363
3	GAPDH	0.2751	GAPDH	0.1768	SamDC	0.2164	GAPDH	0.0738	UBC18	0.156	EF1α	0.3876
4	EF1α	0.2796	ACT7	0.2053	ACT7	0.2434	EF1α	0.1951	GAPDH	0.2276	SamDC	0.4791
5	ACT7	0.3708	TUA6	0.2103	RCA	0.2689	RCA	0.2063	ACT7	0.3401	ACT7	0.4845
6	UBC18	0.5032	UBC18	0.2291	Ubi10	0.3139	Ubi10	0.3426	RCA	0.3682	TUA6	0.5777
7	SamDC	0.5512	EF1α	0.3028	TUA6	0.3279	ACT7	0.3441	Ubi10	0.3776	Ubi10	0.5937
8	RCA	0.8238	SamDC	0.3946	UBC18	0.3309	Ubi4	0.4078	EF1α	0.6029	GAPDH	0.6658
9	TUA6	1.3712	RCA	0.7124	Ubi4	0.8483	TUA6	0.7401	TUA6	0.6579	RCA	0.926

In each column, a low stability value as an estimate of the combined intra- and intergroup variation of the respective gene represents a high gene expression stability of the respective gene between the matched samples.