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. 2008 Oct 15;8:181. doi: 10.1186/1471-2180-8-181

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Copyright © 2008 Jeong et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Bal31 digestion of chromosomal DNA from acid-challeged cells. Bal31 nuclease digestion of chromosomal DNA recovered from acid-stressed cells revealed increased damage in dps, recA, and dps recA strains of E. coli O157:H7. Log-phase cells from the parent strain (ATCC43895), dps (FRIK47992), recA (FRIK4704-kcj05), and dps recA (FRIK4704-kcj06) were acid stressed at pH 2.0 for 2 h before genomic DNA was extracted and quantified. One micro-gram of DNA was digested with Bal31 nuclease and the entire digestion mixture was loaded into an agarose gel. (A) Visualization of ethidium bromide staining. (B) Relative intensity of each sample lane plotted against assigned migration distance.