Table 1.
Strains and plasmids used in this study.
| Strains and plasmid | Relevant characteristicsa | Reference or source |
| Strains | ||
| DH5α | supE44 ΔlacU169 (Φ80 LacZ ΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | Lab collection |
| SY327 λpir | Δ (lac operon) argE(Am) recA56 rpoB λpir; host for π-requiring plasmids | [26] |
| SM0 λpir | thi thr leu tonA lacY supE recA::RP4-2-Tc::Mu λpir, oriT of RP4, Kmr | [38] |
| BL21(DE3) | BL21λ (DE3) under lac control; ompT lon dcm | Novagen |
| ATCC43895 | stxI stxII serotype O157:H7 | Lab collection |
| FRIK47992 | ATCC43895, dps::npt1 | [10] |
| FRIK4704-kcj05 | ATCC43895, ΔrecA | This study |
| FRIK4704-kcj06 | FRIK47992 (ATCC43895, dps, ΔrecA) | This study |
| Plasmids | ||
| pUC4K | pUC4 with nptI; Apr, Kmr | [39] |
| pCVD442 | R6K γori, sacB, oriT of RP4; Apr | [24] |
| pKCJ0325 | pET21b with dps; Apr | This study |
| pKCJ0328 | pCVD442 with alas::mltB; Apr | This study |
aApr, ampicillin resistant; Kmr, kanamycin resistant