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Gene dosage analysis of the SCA20 duplicated region. Results are the mean of three replicates done in triplicate experiments and are expressed as 2−ΔΔCt ± SD. (A) Gene dosage results obtained for the entire SCA20 duplicated region comparing an unaffected family member to an affected family member. The genes outside the duplication are SYT7 and INCENP. (B) A representative gene dosage result for the entire SCA20 pedigree that was tested for one of the duplicated genes, DAGLA exon 5.
