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. Author manuscript; available in PMC: 2009 Jun 1.
Published in final edited form as: J Immunol. 2008 Jun 1;180(11):7461–7470. doi: 10.4049/jimmunol.180.11.7461

FIGURE 3.

FIGURE 3

BAC clone with two partial H chain loci. The 075N10 BAC clone was unique in containing VH gene segments from G5 and Cμ exons from G2A, as identified by Southern blotting (see Table II) and confirmed by sequenced PCR fragments of VH and Cμ2. The 075N10 contained an insert of ~140 kb DNA without internal NotI sites. The Ig genes were mapped in relation to the BAC vector sites NotI and NcoI, present on either side of the vector HindIII cloning site (accession no. U51113). A unique Aat II site 5′ of the G5 VH leader sequence is ~3.2 kb from the vector end. The G2A Cμ1 is present on an 8 kb NcoI fragment that proved to contain 1.3 kb vector after a double digest with NotI. The distance from the HindIII site in the vector to one that is 3′ of the Cμ1 exon is 6 kb. The four C exons are represented as filled boxes, and the VH gene as open box flanked by leader and D, JH gene segments.