Table II.
Restriction endonuclease sites specific to genomic μ loci
| Southern Blotting
|
|||||
|---|---|---|---|---|---|
| VH Probe
|
Cμ1 Probe
|
||||
| Subfamily | Member | BamH I/Nco I | Ava II | Ava II | PCR Cμ2 Product Site Detected |
| G1 | 0.45, 0.7 kb | 1.8 kb | 10 kb | Cla I | |
| G2 | A(V1), B | 1.9 kb | 0.3, 0.37 kb | 0.9 kb | Hind III |
| A (V2) | 1.9 kb | 0.3, 5 kb | 0.9 kb | Hind III | |
| ψC | 1.9 kb | 0.3, 1.1 kb | 0.9 kb | Hind III | |
| G3 | 3 kb | 1 kb, 3.5 kb | 1.5 kb | EcoR I | |
| G4 | B, E, G | 1.9 kb | 1 kb (x2) | 1 kb | EcoR I, Hph I |
| A, C, D | 1.9 kb | 1 kb, 4 kb | 1 kb | EcoR I, Hph I | |
| Fa | 1.9 kb | 2 kb | 1 kb | EcoR I, Hph I | |
| G5 | 1.4, 7.8 kb | 3.9 kb | 0.7 kb | None of the above | |
a
The VH of G1 and G5 carry a BamHI site in FR2; those of G2, G3 and G4 Ava II sites. The VH of G4F is an exception in lacking an internal Ava II and so appears as a single band at 2 kb.
See erratum in: J. Immunol. 2008 Aug 15; 181(4): 2933-4