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. Author manuscript; available in PMC: 2009 Nov 15.
Published in final edited form as: Bioorg Med Chem Lett. 2008 Aug 14;18(22):5948–5950. doi: 10.1016/j.bmcl.2008.08.035

Figure 2.

Figure 2

Fluorescence responses of 5 µM NPF1 to 100 µM reactive oxygen species (ROS). Hydrogen peroxide (H2O2), tert-butyl hydroperoxide (TBHP), and hypochlorite (OCl−) were delivered from 30%, 70%, and 5% aqueous solutions, respectively. Hydroxyl radical (•OH) and tert-butoxy radical (•OtBu) were generated by reactions of 1 mM Fe2+ with 100 µM H2O2 or 100 µM TBHP, respectively. Superoxide (O2−) was generated enzymatically using a xanthine/xanthine oxidase system. NO+ was delivered using S-nitrosocysteine (SNOC). NO was delivered using NOC-5. Spectra were acquired in 20 mM HEPES, pH 7.5, and all data were obtained after incubation with the appropriate ROS at 37 °C. Bars represent relative emission responses (λexc = 598 nm, λem = 660 nm at 0 (white), 15 (light gray), 30 (gray), 45 (dark gray), and 60 min (black) after addition of the appropriate ROS.