Figure 1.

Retina expresses a new splice variant of PCP2 transcript, Ret-PCP2. A, A prominent PCR product of ∼450 bp (arrow) resulted from PCR amplification of retinal (ret) cDNA and ON bipolar (bp) cDNA library. The sequence of this reaction product (Ret-PCP2) is shown in C. Control included water instead of cDNA sample. B, Schematic representation of the three splice variants of PCP2. The filled boxes indicate the coding region. C, Sequence organization of the mouse Pcp2 locus. Ret-PCP2 includes a region upstream to exon 1A (named exon 1ret). Exon 1ret has a start codon that predicts a protein of 15 kDa. The boxed areas indicate Go-Loco motifs. The primers used are highlighted in yellow, and the amino acid sequence used to generate the antibody is highlighted in gray. The 5′ upstream/exon 1ret junction (indicated with red/blue nucleotides) was determined by isolating individual RACE clones and sequencing with a vector primer. Different protein products are represented with different colored single-letter amino acid codes.