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. Author manuscript; available in PMC: 2009 Sep 1.
Published in final edited form as: Proteomics. 2008 Sep;8(18):3906–3918. doi: 10.1002/pmic.200800215

Figure 5.

Figure 5

Inactivation of ATP synthase and mitochondrial complex I activities in MDMA-exposed rat liver mitochondria and immunoblot analysis. (A) Mitochondrial ATP synthase activities in vehicle and MDMA-treated rat livers are presented. *significantly different from the vehicle control (*p< 0.005). (B) Mitochondrial β-ATP synthase from the vehicle and MDMA-exposed rat livers in the absence or presence of 0.5 M sodium dithionite were immunoprecipitated with the specific antibody against β-ATP synthase, separated on 12 % SDS-PAGE, and subjected to immunoblot analysis using the anti-β-ATP synthase antibody (left) or the anti-3-NT antibody (right). This figure represents a typical result from three independent experiments. (C) NADH-ubiquinone oxidoreductase (complex I) activity was measured as described in Materials and Methods. *significantly different from the vehicle control (*p< 0.005).