Table 1.
Summary of LC-MS/MS peptide sequence analyses for oxidized proteins in MDMA treated rat liver mitochondria
| Spot No |
Protein Identified | Swiss-Prot Accession |
Detect No |
|---|---|---|---|
| 1 | 60-kDa Heat shock protein (HSP 60) | P63039 | 79 |
| Stress-70 protein (GRP 75) | P48721 | 11 | |
| 78-kDa Glucose-regulated protein (GRP 78) | P06761 | 5 | |
| 2 | Methylmalonate-semialdehyde dehydrogenase (ALDH6) | Q02253 | 8 |
| Aldehyde dehydrogenase 2, mitochondrial (ALDH2) | P11884 | 3 | |
| Acyl-CoA oxidase 2 | P97562 | 2 | |
| Catalase | P04762 | 2 | |
| Heat shock protein HSP 90-alpha (HSP 86) | P82995 | 2 | |
| 3 | Methylmalonate-semialdehyde dehydrogenase (ALDH6) | Q02253 | 16 |
| Glutamate dehydrogenase 1 (GDH) | P10860 | 9 | |
| Aldehyde dehydrogenase 2, mitochondrial (ALDH2) | P11884 | 2 | |
| 4 | Methylmalonate-semialdehyde dehydrogenase (ALDH6) | Q02253 | 16 |
| Glutamate dehydrogenase 1 (GDH) | P10860 | 14 | |
| UDP-glucose pyrophosphorylase 2 | Q4V8I9 | 5 | |
| Aldehyde dehydrogenase family 7 member A1 (ALDH7) | Q64057 | 3 | |
| Aldehyde dehydrogenase X (ALDH X) | Q66HF8 | 3 | |
| Voltage-dependent T-type calcium channel subunit α-1G (VSCC) | O54898 | 2 | |
| ATP synthase subunit alpha (α-ATP) | P15999 | 2 | |
| 5 | Methylmalonate-semialdehyde dehydrogenase (ALDH6) | Q02253 | 21 |
| Glutamate dehydrogenase 1 (GDH) | P10860 | 12 | |
| UDP-glucose pyrophosphorylase 2 | Q4V8I9 | 8 | |
| ATP synthase subunit alpha (α-ATP) | P15999 | 3 | |
| Aldehyde dehydrogenase family 7 member A1 (ALDH7) | Q64057 | 2 | |
| Aldehyde dehydrogenase X (ALDH X) | Q66HF8 | 2 | |
| Cytochrome P450 11B1 | P15393 | 2 | |
| 6 | Methylmalonate-semialdehyde dehydrogenase (ALDH6) | Q02253 | 16 |
| Glutamate dehydrogenase 1 (GDH) | P10860 | 15 | |
| Cytochrome P450 11B1 | P15393 | 2 | |
| UDP-glucose pyrophosphorylase 2 | Q4V8I9 | 2 | |
| 7 | Methylmalonate-semialdehyde dehydrogenase (ALDH6) | Q02253 | 24 |
| Glutamate dehydrogenase 1 (GDH) | P10860 | 12 | |
| UDP-glucose pyrophosphorylase 2 | Q4V8I9 | 5 | |
| ATP synthase subunit alpha (α-ATP) | P15999 | 3 | |
| Fumarate hydratase | P14408 | 2 | |
| 8 | Methylmalonate-semialdehyde dehydrogenase (ALDH6) | Q02253 | 16 |
| Cytochrome P450 11B1 | P15393 | 3 | |
| Fumarate hydratase | P14408 | 3 | |
| Cytochrome P450 19A1 | P22443 | 2 | |
| 9 | Methylmalonate-semialdehyde dehydrogenase (ALDH6) | Q02253 | 17 |
| Cytochrome P450 11B1 | P15393 | 2 | |
| 10 | 3-Ketoacyl-CoA thiolase | P13437 | 7 |
| Methylmalonate-semialdehyde dehydrogenase (ALDH6) | Q02253 | 6 | |
| Acetyl-CoA dehydrogenase, short chain | Q6IMX3 | 3 | |
| Glutamate dehydrogenase 1 (GDH) | P10860 | 2 | |
| Long-chain specific acyl-CoA dehydrogenase | P15650 | 2 | |
| 11 | 3-Ketoacyl-CoA thiolase | P13437 | 12 |
| Methylmalonate-semialdehyde dehydrogenase (ALDH6) | Q02253 | 8 | |
| Glutamate dehydrogenase 1 (GDH) | P10860 | 4 | |
| Fumarate hydratase | P14408 | 3 | |
| Acetyl-CoA acetyltransferase | P17764 | 2 | |
| Medium-chain specific acyl-CoA dehydrogenase | P08503 | 2 | |
| 12 | 3-Ketoacyl-CoA thiolase | P13437 | 1 |
| 13 | Probable oxidoreductase | Q68FT3 | 4 |
| 3-Ketoacyl-CoA thiolase | P13437 | 3 | |
| Acetyl-CoA dehydrogenase, short chain | Q6IMX3 | 3 | |
| Protein disulfide-isomerase A6 precursor | Q63081 | 3 | |
| 3-Ketoacyl-CoA thiolase A, peroxisomal | P21775 | 2 | |
| Peroxisomal delta3, delta2-enoyl-CoA isomerase | Q5XIC0 | 2 | |
| 14 | Acetyl-CoA dehydrogenase, short chain | Q6IMX3 | 4 |
| 3-Ketoacyl-CoA thiolase | P13437 | 3 | |
| Peroxisomal delta3, delta2-enoyl-CoA isomerase | Q5XIC0 | 3 | |
| Glutamate dehydrogenase 1 (GDH) | P10860 | 2 | |
| Methylmalonate-semialdehyde dehydrogenase (ALDH6) | Q02253 | 2 | |
| 15 | 60-kDa Heat shock protein (HSP 60) | P63039 | 4 |
| 16 | 60-kDa Heat shock protein (HSP 60) | P63039 | 10 |
| ATP synthase subunit beta (β-ATP) | P10719 | 2 | |
| 17 | Glutamate dehydrogenase 1 (GDH) | P10860 | 4 |
| Methylmalonate-semialdehyde dehydrogenase (ALDH6) | Q02253 | 2 | |
| 18 | Enoyl-CoA hydratase | P14604 | 6 |
| 19 | Enoyl-CoA hydratase | P14604 | 9 |
| Glutamate dehydrogenase 1 (GDH) | P10860 | 2 | |
| 20 | Electron transfer flavoprotein subunit beta (β-ETF) | Q68FU3 | 9 |
| 3-Hydroxyacyl-CoA dehydrogenase 2 | O70351 | 7 | |
| Enoyl-CoA hydratase | P14604 | 6 | |
| Glutamate dehydrogenase 1 (GDH) | P10860 | 5 | |
| 21 | Enoyl-CoA hydratase | P14604 | 4 |
| Glutamate dehydrogenase 1 (GDH) | P10860 | 2 | |
| 22 | Enoyl-CoA hydratase | P14604 | 5 |
| Heat shock protein 75 kDa (HSP 75) | Q5XHZ0 | 4 | |
| 3-Hydroxyacyl-CoA dehydrogenase 2 | O70351 | 3 | |
| 23 | Enoyl-CoA hydratase | P14604 | 5 |
| 24 | Enoyl-CoA hydratase | P14604 | 5 |
| 25 | Enoyl-CoA hydratase | P14604 | 2 |
| 26 | ATP synthase subunit alpha (α-ATP) | P15999 | 14 |
| Glutamate dehydrogenase 1 (GDH) | P10860 | 2 | |
| 27 | ATP synthase subunit alpha (α-ATP) | P15999 | 8 |
| Methylmalonate-semialdehyde dehydrogenase (ALDH6) | Q02253 | 2 | |
| 28 | ATP synthase subunit alpha (α-ATP) | P15999 | 9 |
| 29 | ATP synthase subunit alpha (α-ATP) | P15999 | 6 |
| ATP synthase D chain | P31399 | 2 | |
| 30 | ATP synthase subunit alpha (α-ATP) | P15999 | 7 |
| 31 | Glutamate dehydrogenase 1 (GDH) | P10860 | 3 |
| Methylmalonate-semialdehyde dehydrogenase (ALDH6) | Q02253 | 3 | |
| 32 | Methylmalonate-semialdehyde dehydrogenase (ALDH6) | Q02253 | 2 |
| 33 | 60-kDa Heat shock protein (HSP 60) | P63039 | 21 |
| Cytochrome b5 | P00173 | 5 | |
| Glutamate dehydrogenase 1 (GDH) | P10860 | 2 | |
| 10-kDa Heat shock protein (HSP 10) | P26772 | 2 | |
| DNA polymerase subunit gamma 1 (Mitochondrial DNA polymerase catalytic subunit) |
Q9QYV8 | 2 |
Biotin-NM labeled oxidized proteins were isolated with streptavidin-agarose, washed, resolved on 2-D gels, and stained with silver. Each protein spot as indicated was picked up with a razor blade and subjected to protein identification using mass spectrometric analysis, as described in the Experimental Procedures and Supplementary Table 1.