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. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: J Heart Lung Transplant. 2008 Aug 27;27(10):1142–1149. doi: 10.1016/j.healun.2008.07.006

Fig. 3.

Fig. 3

The effects of Trx priming of donor lungs on NF-kB/DNA binding activity in native and allograft transplants. Donor lungs were primed with an infusion of Perfadex with or without (control) Trx (4 µg/ml) for 4 hr as described in Materials and Methods. Transplanted and native lungs were isolated one and five days post-transplant. NF-kB/DNA binding activity was monitored using an oligonucleotide representing specific NFkB binding sequences on the Trx gene. Lanes 1 and 2 = native lungs; Lanes 3 and 4 = one and five days post-transplant allografts; and Lanes 5 and 6 = Trx-primed one and five days post-transplant allografts. Lanes 1, 3, and 5 = one day post transplant, and Lanes 2, 4, and 6 = five day post-transplant allografts. Data shown are from one of two gel shift blots with similar results.