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. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: Arch Biochem Biophys. 2008 Jul 16;478(1):18–25. doi: 10.1016/j.abb.2008.07.003

Figure 4. Characterization of the preparations of fusion proteins containing wild-type or mutant Gal1 by SDS-PAGE.

Figure 4

Lane 1: GST; lane 2, GST-Gal1(WT); lane 3, GST-Gal1(N46D); lane 4, GST-Gal1(C60S); and lane 5, GST-Gal1 (E71Q). The proteins (~30 ng in each lane) were electrophoresed through 12.5% acrylamide gels. Panel A: silver staining; Panel B: immunoblotting with affinity purified anti-Gal1 antibodies (#55); and Panel C: immnoblotting with affinity purified anti-GST antibodies. The positions of migration of molecular weight standards are indicated on the left.