Skip to main content
. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: Arch Biochem Biophys. 2008 Jul 16;478(1):18–25. doi: 10.1016/j.abb.2008.07.003

Figure 5. Comparison of the carbohydrate-binding activity of GST-Gal1(WT), GSTGal1(N46D), GST-Gal1(C60S), and GST-Gal1(E71Q).

Figure 5

The GST proteins were incubated with ASF-Sepharose for 2 hours at 4 °C in 60% buffer D containing 0.1% NP-40. Parallel incubations were carried out in the presence and absence of 100 mM Lac. Proteins bound to the ASF-beads were quantitated by immunoblotting with anti-GST. The values shown represent Lac inhibitable specific binding.