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. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: Arch Biochem Biophys. 2008 Jul 16;478(1):18–25. doi: 10.1016/j.abb.2008.07.003

Figure 6. Comparison of the GST pull-down of TFII-I in NE by wild-type and mutant Gal1 polypeptides.

Figure 6

The GST proteins were incubated overnight with glutathione beads at 4 °C in 60% buffer D. After washing to remove the unbound material, the beads were then incubated with NE for 12 hours at 4 °C. Material bound to the various beads was eluted with glutathione (16 mM) and analyzed by SDS-PAGE and immunoblotting. Panel A: immunoblotting with anti-TFII-I (# 558). Panel B:immunoblotting with anti-GST to ascertain that approximately equal amounts of GST proteins were bound to the beads in the pull-down assay.