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. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: Arch Biochem Biophys. 2008 Jul 16;478(1):18–25. doi: 10.1016/j.abb.2008.07.003

Figure 8. Comparison of the effects of GST-Gal1(WT) and GST-Gal1(N46D) on the splicing activity of NE containing TDG.

Figure 8

Lane 1, TDG addition (150 mM); Lane 2, cellobiose addition (150 mM); Lanes 3–5, TDG (150 mM) and GST-Gal1(WT) (10, 20, and 30 µM); Lanes 6–8, TDG (150 mM) and GST-Gal1( N46D) (10, 20, and 30 µM). Products of the splicing reactions were analyzed by electrophoresis through a 13% polyacrylamide-urea gel and autoradiography. The positions of migration of the pre-mRNA substrate, the splicing intermediates, and the mature RNA product are indicated on the right.