Colocalization of FGD2 with organelle markers reveals partial
localization to early endosomes and membrane ruffles. Shown are confocal
fluorescent microscopy images of HeLa cells transfected with EGFP-FGD2 prior
to fixation, permeabilization, and staining with antibodies against the
endosomal marker EEA1 (upper panels), the lysosomal marker LAMP2
(middle panels), the cis-Golgi marker GM130 (lower
panels) and phalloidin, a stain for F-actin (bottom panels).
Bound antibody was detected with anti-mouse IgG Alexa 568 (red). A
10-μm scale is shown as white bars.