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. 2008 Dec 5;283(49):34002–34012. doi: 10.1074/jbc.M803957200

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Copyright © 2008, The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — Recombinant FGD2 directly binds to phospholipids. His-thioredoxin protein alone (TRX; left) or His-thioredoxin-FGD2 fusion protein (TRX-FGD2; right) was incubated with membranes spotted with listed concentrations of phospholipids. After washing, bound protein was detected with an anti-His antibody and chemiluminescence. PI, phosphatidylinositol; PI(3)P, phosphatidylinositol-3-phosphate; PI(4)P, phosphatidylinositol-4-phosphate; PI(5)P, phosphatidylinositol-5-phosphate; PI(3,4)P2, phosphatidylinositol 3,4-bisphosphate; PI(3,5)P2, phosphatidylinositol 3,5-bisphosphate; PI(4,5)P2, phosphatidylinositol 4,5-bisphosphate; PI(3,4,5)P3, phosphatidylinositol 3,4,5-trisphosphate.