FIGURE 8.

Insulin prevents pericyte apoptosis induced by H₂O₂ via IRS1/PI3K pathway. BRPC were seeded in a 6-well culture plate, incubated with insulin (100 nm) in absence or presence of wortmannin (100 nm) or zinc protoporphyrin (10 μm), and then treated with H₂O₂ for 1 h as described under “Experimental Procedures” (A). BRPC were transfected with control GFP, wild-type IRS1 or IRS2, and exposed to insulin and/or H₂O₂ (B). DNA fragmentation was measured according to the manufacturer's instructions. Each value was normalized by the untreated cell response. The results are shown as the means ± S.D. of three independent experiments. ^*, p < 0.01 versus PBS-treated cells; †, p < 0.05 versus H₂O₂.