FIGURE 9.

Reduction of NF-κB binding activity induced by H₂O₂ by insulin through IRS1. BRPC were seeded in a 6-well culture plate, transfected with control GFP, wild-type IRS1, or IRS2, incubated in the absence or presence of insulin (100 nm) for 12 h, and then exposed to H₂O₂ for 1 h as described under “Experimental Procedures.” NF-κB binding activity assay was performed according to the manufacturer's instructions as described under “Experimental Procedures.” The results are shown as the means ± S.D. of three independent experiments. ^*, p < 0.001 versus PBS-treated cells; †, p < 0.01 versus H₂O₂.