TABLE 1.
Respiratory analysis of WT and p66Shc−/− BAT isolated mitochondria
The average values and S.D. of three independent experiments are indicated.
Isolated MTC
|
|||||
---|---|---|---|---|---|
+Fat free BSA
|
w/o fat free BSA
|
||||
WT | p66−/− | WT | p66−/− | ||
nmol/ml/mg of protein | |||||
Basal | 11.76 ± 2.43 | 9.14 ± 3.55 | 6.95 ± 1.86 | 6.14 ± 0.40 | |
Pyruvate/malate | 36.12 ± 5.24 | 35.10 ± 6.78 | 52.68 ± 6.13a | 64.24 ± 5.37a | |
Glycerol 3-phosphate | 50.61 ± 5.94 | 46.82 ± 5.30 | 70.32 ± 4.51b | 96.15 ± 7.16b | |
GDP | 35.23 ± 6.36 | 36.51 ± 6.27 | 39.71 ± 5.18 | 42.07 ± 4.23 | |
FCCP | 102.34 ± 8.12 | 113.45 ± 9.26 | 108.22 ± 9.41 | 104.26 ± 8.51 | |
Palmitate | 79.24 ± 8.19b | 102.11 ± 9.03b | |||
ADP | 94.56 ± 8.22 | 110.07 ± 7.48 |
p < 0.05.
p < 0.01.