TABLE 2.

Summary of the single nucleotide incorporation kinetic constants for HIV-RT at position n + 1 and n + 4 K_m is in μm dTTP or dCTP. k_cat is in min^–1. -Fold change reflects -fold differences between k_cat/K_m with n = dGMP and k_cat/K_m with n = ETV-MP. S.D. was determined on the basis of at least three independent experiments.

Site	Nucleotide	Primer						Change
				n = dGMP			n = ETV-MP
		kcat	Km	kcat/Km	kcat	Km	kcat/Km
		min–1	μm		min–1	μm		-fold
n + 1	dTTP	0.38 ± 0.070	0.057 ± 0.0074	6.7	0.36 ± 0.057	0.38 ± 0.035	0.95	7
n + 4	dCTP	0.30 ± 0.011	0.10 ± 0.017	3.0	0.076 ± 0.013	33 ± 8.3	0.0023	1300