Skip to main content
. 2008 Dec 5;283(49):34129–34140. doi: 10.1074/jbc.M806668200

TABLE 1.

Effect of POL32 and PIF1 deletions on the viability of dna2Δ

DNA2 allele
Number of spores with a genotypea
Totalb
PIF1+POL32+ pif1Δ POL32+ pif1Δ pol32Δ PIF1+pol32Δ
DNA2+ 38 56 43 64 201
dna2Δ 0 57 43 3c 104
a

Presented are numbers of each genotype within the haploid progeny of two isogenic diploids of the genotype: DNA2+/dna2Δ PIF1+/pif1Δ POL32+/pol32Δ

b

Plates after dissection were incubated at 23 and 30 °C. The haploid progeny grew equally well at either temperature, so the numbers were combined in this table

c

The genotype of three rare dna2Δ pol32Δ PIF1+ segregants was confirmed by PCR