TABLE 1.
DNA2 allele
|
Number of spores with a
genotypea
|
Totalb
|
|||
---|---|---|---|---|---|
PIF1+POL32+ | pif1Δ POL32+ | pif1Δ pol32Δ | PIF1+pol32Δ | ||
DNA2+ | 38 | 56 | 43 | 64 | 201 |
dna2Δ | 0 | 57 | 43 | 3c | 104 |
Presented are numbers of each genotype within the haploid progeny of two isogenic diploids of the genotype: DNA2+/dna2Δ PIF1+/pif1Δ POL32+/pol32Δ
Plates after dissection were incubated at 23 and 30 °C. The haploid progeny grew equally well at either temperature, so the numbers were combined in this table
The genotype of three rare dna2Δ pol32Δ PIF1+ segregants was confirmed by PCR