FIGURE 8.
NA large loop mutant C93/419S* accumulates monoglucosylated glycans. A, schematic demonstrating the carbohydrates that are sensitive to endomannosidase digestion. The unglucosylated glycan opens up this branch for the removal of an additional three mannoses by α-mannosidase digestion, producing an accentuated shift in electrophoretic mobility of the treated proteins. B, NA WT* and C93/419S* were overexpressed in CHO cells and radiolabeled with [35S]Met/Cys for 5 min followed by chases of 0 or 30 min. NA purified with Ni-NTA was subjected to JBM digestion.