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. 2008 Dec 5;283(49):33816–33825. doi: 10.1074/jbc.M807428200

FIGURE 2.

FIGURE 2.

Age-dependent changes in chemical and physical properties of glycogen in Epm2a–/– mice. A, iodine spectra of skeletal muscle glycogen (50–75 μg/ml) purified from wild type (WT) and Epm2a–/– mice. Amylopectin is shown for reference. B, quantitation of A.*, p < 0.002 versus wild type; #, p < 0.0001 versus 3-month-old Epm2a–/– (n = 3–8). C, skeletal muscle glycogen (5 mg/ml) purified from a 9-month-old Epm2a–/– mice that was treated with catalytically inactive laforin (C266S) (tube i) or active laforin (tube ii), and brought to 66% (v/v) ethanol. Tube iii, addition of LiCl to tube i. Tube iv, addition of LiCl to tube ii. Tube v, addition of LiCl to a 66% ethanol solution without glycogen.