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. 2008 Dec 5;283(49):33816–33825. doi: 10.1074/jbc.M807428200

FIGURE 5.

FIGURE 5.

Fractionation of glycogen from 9–12-month-old Epm2a–/– mice. Total skeletal muscle or brain extracts from 9–12-month-old wild type (WT) and Epm2a–/– mice were subjected to low speed centrifugation to generate supernatant (LSS) and pellet (LSP). A, percentage of total muscle glycogen, in the LSP. *, p < 0.05 (n = 3). B, percentage of total brain glycogen in the LSP. *, p < 0.005 (n = 3). C, electron micrograph of glycogen purified from the LSS of a muscle extract. D, electron micrograph of glycogen purified from the LSP of a muscle extract. E, skeletal muscle glycogen phosphate levels in LSS and the LSP. #, p = 0.002 (n = 6). F, iodine spectra of muscle glycogen (50–75 μg/ml) (purified from the LSS and the LSP; n = 6). Amylopectin is shown for reference.