FIGURE 4.
Sec7 amino acids 1836–1883 are sufficient for interaction with the C2 domain of Rsp5. A, schematic of Sec7 C terminus fragments. The ability of each fragment to bind the C2 domain is indicated on the right. B, yeast lysates containing GFP-C2 (LHY4488) or GFP-C23K→Q (LHY4007) were incubated with immobilized His6-Sec7 fragments. Bound proteins were eluted by boiling, resolved by SDS-PAGE, and analyzed by immunoblotting with GFP antibodies. The presence of equal amounts of immobilized proteins in each reaction was verified by SDS-PAGE and Coomassie staining (not shown).