Figure 6.

Transgenic ABI5 Expression in rgl2 Mutant Seeds Restores Wild-Type–Like Germination Responses under Low-GA Conditions.

(A) PAC-treated rgl2-1/35S:HA-ABI5, rgl2-1, and wild-type (Ler) plant material at 96 h after seed imbibition (PAC, 5 μM). Percentage of germination on PAC at 96 h is indicated.

(B) Protein gel blot analysis of HA-ABI5 and endogenous ABI5 protein levels in the plant material shown in (A). HA-ABI5 and endogenous ABI5 protein were detected using antibodies against ABI5. Numbers indicating treatment are as in (A). Ponceau S staining is shown as a loading control.