Figure 7.

rgl2 Mutant Seeds Have Low Endogenous ABA and XERICO mRNA Levels under Low-GA Conditions.

(A) Time course of endogenous ABA levels in wild-type (Col) and rgl2-13 seeds under normal conditions and in the presence of PAC (5 μM). Units are nanograms of ABA per gram of dry seed weighed prior to imbibition. Error bars indicate sd (n = 3).

(B) Protein gel blot analysis of the time course of ABI5 protein levels in wild-type (Col) and rgl2-13 mutant seeds under normal conditions and in the presence of PAC (5 μM). Each lane contained protein extract from 100 seeds from the material used in (A). Percentage germination at each time point is indicated. Ponceau S staining is shown as a loading control.

(C) RNA gel blot analysis of the time course of XERICO mRNA accumulation in wild-type (Col) and rgl2-13 mutant seeds under normal and low-GA conditions. Plant material was as in (A) and (B). rRNA is shown as a loading control.