Figure 9.

RGL2 mRNA and Protein Levels Increase More Relative to Those of RGA and GAI under Low-GA Conditions.

(A) RNA gel blot analysis of the time course of RGL2, GAI, and RGA mRNA levels in wild-type (Col) seeds under normal, PAC, and ABA conditions. Two micrograms of total RNA was used per lane. DS, dry seeds. The RNA gel blot images for RGL2 are repeated from Figure 1 for ease of comparison. rRNA is shown as a loading control.

(B) Protein gel blot analysis of the time course of RGL2, GAI, and RGA protein levels in wild-type seeds under normal and low-GA conditions (PAC). Each lane contained protein extract from 100 seeds from the material used in (A) (see Supplemental Figure 15B online for GAI and RGA antibody specificity). Ponceau S staining is shown as a loading control.