Table 1.
Characterization of Rice Mutants and Transgenic Rice Overexpressing GA2oxs
| Traits | Wild Type | GA2ox5Δ335-341ACT (T3)a | GA2ox9ACT (T3) | GA2ox6ACT (T2) | Ubi:GA2ox5 (T1) | Ubi:GA2ox6 (T1) |
|---|---|---|---|---|---|---|
| Tiller number of seedling (18 DAI) | 1.0 ± 0.0b (100)c | 1.8 ± 0.8 (180) | 1.0 ± 0.0 (100) | 2.6 ± 0.5 (260) | 2.7 ± 0.6 (270) | 2.5 ± 0.7 (250) |
| Root length (cm) at 18 DAI | 6.3 ± 0.9 (100) | 15.7 ± 3.2 (249) | 11.0 ± 1.9 (175) | 5.8 ± 1.8 (92) | 6.3 ± 2.1 (100) | 6.6 ± 0.4 (105) |
| Plant height (cm) at 120 DAI | 109.5 ± 2.5 (100) | 98.0 ± 7.1 (90) | 83.2 ± 4.1 (76) | 16.6 ± 1.7 (15) | 16.7 ± 2.8 (15) | 12.1 ± 2.7 (11) |
| Length of leaf bellow flag leaf (cm) at 120 DAI | 49.9 ± 6.0 (100) | 49.6 ± 5.1 (100) | 49.3 ± 3.3 (99) | 12.2 ± 0.9 (24) | 10.6 ± 1.2 (21) | 8.1 ± 0.8 (16) |
| Width of leaf bellow flag leaf (cm) at 120 DAI | 1.64 ± 0.1 (100) | 1.66 ± 0.1 (101) | 1.75 ± 0.2 (107) | 1.51 ± 0.1 (92) | 1.2 ± 0.1 (73) | 1.04 ± 0.1 (63) |
| Heading day (DAI) | 108.7 ± 1.5 | 107.6 ± 1.3 | 107.9 ± 1.0 | > 150 | >150 | >150 |
| Panicle length (cm) | 21.6 ± 2.0 (100) | 20.3 ± 1.5 (94) | 19.7 ± 1.8 (91) | 7.7 ± 1.6 (36) | 5.9 ± 0.8 (27) | 7.5 ± 0.9 (35) |
| Total Tiller number | 11.0 ± 1.8 (100) | 20.3 ± 4.1 (185) | 13.4 ± 2.9 (122) | 17.6 ± 3.7 (160) | NA | 18.8 ± 4.5 (171) |
| Grain weight (g/100 grains) | 2.44 ± 0.1 (100) | 2.04 ± 0.1 (84) | 2.34 ± 0.2 (96) | 1.54 (63) | 1.43 (59) | 1.98 (81) |
| Fertility (%) | 92.6 ± 4.2 (100) | 81.1 ± 5.4 (88) | 85.4 ± 8.9 (92) | 39.5 ± 18 (43) | 27.7 ± 12.0 (30) | 59.4 ± 4.4 (64) |
a
T1, T2, and T3 in parenthesis indicate generation of mutants.
b
se; n = 20 for GA2ox5Δ335-341ACT, GA2ox9ACT, and GA2ox6ACT; n = 10 for Ubi:GA2ox5 and Ubi:GA2ox6.
c
Values in parentheses indicate % of the wild type. NA: not available. DAI: days after imbibition.