Figure 7.
OTS1 and OTS2 Are Nuclear Proteins.
Tobacco leaves were transiently transformed with vectors expressing the following fluorescent proteins: sGFP (A), sGFP:OTS1 (B), sYFP (C), and sYFP:OTS2 (D). For each construct, different fluorescent emissions were collected.
(A) sGFP protein localization at low resolution ([A1] to [A4]) and high resolution ([A5] to [A8]). (A1) and (A5), sGFP emission (green); (A2) and (A6), chloroplast autofluorescence (red); (A3) and (A7), bright field; (A4) and (A8), merged images of (A1) to (A3) and (A5) to (A7), respectively.
(B) sGFP:OTS1 protein fusion localization at low resolution ([B1] to [B4]) and high resolution ([B5] to [B8]). (B1) and (B5), sGFP emission (green); (B2) and (B6), chloroplast autofluorescence (red); (B3) and (B7), bright field; (B4) and (B8), merged images of (B1) to (B3) and (B5) to (B7), respectively.
(C) sYFP protein localization at low resolution ([C1] to [C4]) and high resolution ([C5] to [C8]). (C1) and (C5), chloroplast autofluorescence (red); (C2) and (C6), sYFP emission (yellow); (C3) and (C7), bright field; (C4) and (C8), merged images of (C1) to (C3) and (C5) to (C7), respectively.
(D) sYFP:OTS2 protein fusion localization at low resolution ([D1] to [D4]) and high resolution ([D5] to [D8]). (D1) and (D5), chloroplast autofluorescence (red); (D2) and (D6), sYFP emission (yellow); (D3) and (D7), bright field; (D4) and (D8), merged images of (D1) to (D3) and (D5) to (D7), respectively.