Figure 5.

Isolation of hpr2 Knockout Mutants.

(A) The structure of the HPR2 gene and positions of the T-DNA insertions in hpr2-1 and hpr2-2.

(B) HPR2-specific transcripts are undetectable in both homozygous mutants by RT-PCR analysis of whole-leaf RNA extracts using the S16 RNA amplicon as an internal control and Col-0 wild-type leaf mRNA for comparison.

(C) and (D) HPR2 (C) and HPR1 (D) proteins are undetectable by protein gel blotting in their respective mutants. This experiment shows the results for hpr1-1 and hpr2-1, which are identical to those obtained with hpr1-2 and hpr2-2.