Figure 10.

Escape from Far-Red Reversal of the Red Light Effect on Blue Light–Induced PHOT1-GFP Relocalization.

Projection images of PHOT1-GFP in hypocotyl cortical cells from etiolated 3-d-old Arabidopsis seedlings. Light sources and irradiation protocols as for Figure 9 except that the time between red light (R) and far-red (FR) pulses was varied from 3 to 60 min. t1, time, min, between red and far-red pulses; t2, time, min, between far-red pulse and beginning of blue light irradiation. t1 + t2 = 120 min. A time course for the B effect is shown for each red/far-red treatment. Note the strong blue light response at 3 and 15 min between red and far red, indicating far-red reversal of the red effect. At 25 and 60 min between red and far-red pulses, the red effect is only weakly reversed. Bars = 20 μm.