Restriction of the Red Light Effect on Blue Light–Induced PHOT1-GFP Relocalization to the Hypocotyl Elongation Zone.
Projection images of cortical cells from the hypocotyl hook (A), the elongation zone (B), and mature hypocotyl zones (C) of etiolated 3-d-old Arabidopsis seedlings. Red light fluence rate 10 μmol m−2 s−1, an exposure time of 10 s followed by a 2-h dark incubation period prior to blue light treatment. The blue light source (20 μmol m−2 s−1) is the laser from the confocal microscope. Note that red light treatment eliminates the blue light effect only in the elongation zone. Bars = 20 μm.