Duration of Dark Incubation Period after Red Light Treatment Required for Red Light–Induced Inhibition of Blue Light–Induced PHOT1-GFP Relocalization.
Projection images of hypocotyl cortical cells from 3-d-old etiolated Arabidopsis seedlings. Red-light (R) treatment as in Figure 1B. Blue light (B) treatment as in Figure 4. Dark incubation times were 0, 30, 60, 120, and 180 min. A maximum effect of red light in preventing blue light–induced PHOT1-GFP relocalization is seen only after 2 or 3 h of dark incubation. Bars = 20 μm.