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. 2008 Oct;20(10):2746–2762. doi: 10.1105/tpc.108.059048

Figure 1.

Figure 1.

Conditional Repression of ABP1 Activity in Arabidopsis.

(A) and (B) Expression kinetics of scFv12 (A) and ABP1 antisense (B) mRNA accumulation after exposure to ethanol in AtSS12K and AtABP1AS lines, respectively. Data are expressed as the ratio between induced and noninduced samples.

(C) Immunodetection of the recombinant antibody scFv12 in a microsomal fraction of wild-type and AtSS12K plants induced with ethanol for 24 h. Top blot shows signal detected with an antibody against scFv12. Bottom panel shows protein loading detected with Ponceau S staining prior to incubation with the specific antibody.

(D) Immunodetection of ABP1 in enriched microsomal fractions from wild-type and AtABP1AS plants induced with ethanol for 3 d. Top blot shows signal detected with an antibody against ABP1. Bottom blot shows protein loading detected with Ponceau S staining prior to incubation with the specific antibody.

(E) to (G) Apices of wild-type (E), AtSS12K (F), and AtABP1AS (G) 5-d-old seedlings grown in vitro in the presence of 5% ethanol vapor. Cotyledon epinasty and decreased leaf growth are apparent in (F) and (G) compared with (E). Bar = 2.5 mm.