Figure 9.

ABA Can Suppress Cytokinin-Induced Nodulation Responses.

(A) Treatment with 0.01 μM cytokinin (BAP) for 10 d induces ENOD40 in M. truncatula, and this is blocked upon treatment with ABA. sta-1 shows increased levels of ABA suppression of ENOD40 induction by cytokinin.

(B) Treatment with 0.01 μM BAP induces NIN in M. truncatula, and this is blocked upon treatment with ABA. sta-1 shows increased levels of ABA suppression of NIN induction by cytokinin.

(C) Wild-type M. truncatula plants were transformed with abi1-1 or empty vector (EV) and tested for cytokinin (BAP) induction of ENOD40. abi1-1–transformed plants showed much higher levels of ENOD40 induction by cytokinin compared with EV transformed plants.

For (A) and (B), 10 plants were used for each treatment and four biological repeats were performed. All error bars represent se.