Table 3.
Bax mRNA Levels Increase After Traumatic Brain Injury
| BRAIN REGION | MODEL | TIME POSTINJURY (days) | ||||
|---|---|---|---|---|---|---|
| SHAMS | 0.25 | 1 | 3 | 7 | ||
| CORTEX | bcl-xL mRNA (fg/μg protein)a | |||||
| IPSILATERAL | LFP | 5.27±0.51 | - | 6.67±0.49 | 7.45±0.85* | 7.44±0.46* |
| LCI | 2.04±0.25 | 2.81±0.32§ | 1.92±0.35 | 1.57±0.38 | 1.28±0.17 | |
| CONTRALATERAL | LFP£ | 4.39±0.42 | - | 5.76±0.67# | 5.55±0.14^ | 3.21±0.44# |
| |
LCI£ |
2.91±0.56^ |
2.40±0.38 |
2.35±0.29 |
2.43±0.65^ |
1.71±0.26 |
| HIPPOCAMPUS | ||||||
| IPSILATERAL | LFP | 1.68±0.27 | - | 1.86±0.17 | 2.59±0.30* | 2.40±0.20* |
| LCI | 3.45±0.86 | 2.74±0.27 | 2.76±0.80 | 5.69±0.62* | 7.25±1.35* | |
| CONTRALATERAL | LFP | 1.69±0.20 | - | 1.62±0.25 | 2.18±0.57 | 2.13±0.12 |
| LCI | 5.28±0.71 | 5.65±1.06# | 4.86±0.91 | 4.38±0.95# | 6.18±1.43 | |
a
Messenger RNA levels (mean ± SEM) determined by RNase protection assay (see Methods). Sham controls from multiple time points showed no differences and were combined for each anatomical area. Lateral fluid percussion (LFP) or lateral cortical impact (LCI) injury was performed as described in Methods. “-” not done.
*
p<0.05, ANOVA, Dunnett vs. shams
§
p<0.05, ANOVA, Fisher PLSD vs. shams.
#
p<0.05, paired t-test, contralateral vs. ipsilateral
^
0.05<p<0.10
£
p<0.05, paired t-test, contralateral vs. ipsilateral over time groups combined.